Sexual Determination and Differentiation

 

Differentiation of genitalia

differentiation = anatomical development; process by which cells develop (differentiate) into specific cell types.

 

Developmental Event/Condition

Normal Female Development

Normal Male Development

Chromosomal Sex

 

 

 

Presence of TDF gene?

 

 

 

Anlagen for gonads

bipotential germinal ridge

 

Expression of TDF gene?

 

 

 

Gonad Development

 

 

 

Gonad Hormone Production

 

 

 

 

Anlagen for male accessory organs

Wolfian duct

 

Wolfian duct exposure to testosterone?

 

 

Wolfian duct development?

 

 

 

Anlagen for female accessory organs

Mullerian duct

 

Mullerian duct exposure to MIH?

 

 

Mullerian duct development?

 

 

 

Anlagen for external genitalia

Genital skin containing 5-alpha-reductase

 

 

Genital skin exposure to DHT?

 

 

External genitalia development

 

 

 

Anlagen — primordial tissue: the first accumulation of cells in an embryo which constitutes the beginning of a future tissue or organ.

MIH = Mullerian Inhibitory Hormone (AKA Mullerian Regression Factor)

DHT = 5-alpha-dihydrotestosterone

What enzyme catalyzes the conversion of testosterone to the Òsuper testosteroneÓ, 5-alpha-dihydrotestosterone (DHT)?

 

Propecia (finasteride): mechanism of action—

 

                  potential developmental side effects—

 

 

Sexual differentiation is a demonstration of organizational effect of hormones.  If after birth a male is castrated the genitalia will remain masculine.  It also illustrates critical periods—no amount of testosterone after differentiation of these structures along female lines will convert them into the masculine form.

 

Sexual Anomalies (see Table)

 

Male (XY) lack of exposure to TDF, MIH, testosterone or DHT during critical periods of development:

 

TFM (AKA androgen insensitivity):

 

      side note: chromosomal location of androgen receptor—

 

      Secondary sex characteristics

 

            Breast development—

 

            Pubic hair—

 

            LH, FSH levels—

 

            Testosterone levels—

 

            incidence—

 

 

5-alpha-reductase deficiency:

 

      Gender assignment at birth—

 

      Gender identity—

 

 

 


 

 

 

 

 

 

 

Chromosome

(Total #, Sex)

Syndrome/De-fect

gonads

accessory sexual organs

external genitalia

secondary sex characteristics

 

46, XY

 

 

Lack of TDF

 

 

 

 

 

 

 

 

 

 

 

46, XY

Testicular Feminization Mutation (TFM); absence of androgen receptors

 

 

 

 

 

 

 

 

 

 

 

46, XY

Lack of MIH or defective MIH receptors

 

 

 

 

 

 

 

 

 

 

 

46, XY

5-alpha-reductase deficiency (guevedoces)

 

 

 

 

 

 

 

 

 

 

 

46, XX

Fetal exposure to exogenous steroids with androgen effects

 

 

 

 

 

 

 

 

 

 

 

46, XX

Fetal exposure to endogenous androgens (Congenital Adrenal Hyperplasia)

 

 

 

 

 

 

 

 

 

 

 

45, XO

Turner's syndrome

(monosomy)

 

 

 

 

 

 

 

 

 

 

 

 

47, XXY

Klinefelter syndrome (trisomy)

 

 

 

 

 

 

 

 

 

 

 

47, XYY

trisomy